Development of a Microfluidic System for Mitochondrial Extraction, Purification, and Analysis

Mitochondria, as essential cellular organelles, play a key role in numerous diseases, from neurodegenerative disorders to cancer and rare conditions. The extraction of mitochondria from cells has many applications in disease diagnosis, pathological research, and emerging mitochondrial transplantation therapy (MTT). Recent advancements in microfluidic-on-chip systems offer promising improvements in mitochondrial extraction by enabling high-throughput processing, precise control, and flexibility while facilitating integration with other devices and platforms. Despite growing interest in microfluidic mitochondrial extraction (MME), there is a lack of comprehensive reviews on the latest developments in this field. This review aims to summarize recent advancements as well as the advantages and limitations of MME, providing deeper insights into microfluidic-based approaches for mitochondrial extraction, purification, and analysis.