Design, synthesis and application of fluorogenic probe for detecting L-asparaginase in serum samples

Lan Wang; Wenjing Qin; Ding Chen; Nanxiang Wang; Congcong Zhang; Zhijie Fang; Bin Fang; Wei Du; Naidi Yang; Qiong Wu; Bo Peng; Lin Li

doi:10.1016/j.rechem.2021.100103

Design, synthesis and application of fluorogenic probe for detecting L-asparaginase in serum samples

Lan Wang
, Wenjing Qin
, Ding Chen
, Nanxiang Wang
, Congcong Zhang
, Zhijie Fang
, Bin Fang
, Wei Du
, Naidi Yang
, Qiong Wu
, Bo Peng
, Lin Li

Institute of Flexible Electronics

Nanjing Tech University
Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro L-asparaginase detection. TPAN-Asn exhibited selective and robust response to L-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of L-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of L-asparaginase-based cancer therapy.

Original language	English
Article number	100103
Journal	Results in Chemistry
Volume	3
DOIs	https://doi.org/10.1016/j.rechem.2021.100103
State	Published - Jan 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Fluorogenic probe
In vitro assay
L-asparaginase
Patient serum

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10.1016/j.rechem.2021.100103

Cite this

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title = "Design, synthesis and application of fluorogenic probe for detecting L-asparaginase in serum samples",

abstract = "Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro L-asparaginase detection. TPAN-Asn exhibited selective and robust response to L-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of L-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of L-asparaginase-based cancer therapy.",

keywords = "Fluorogenic probe, In vitro assay, L-asparaginase, Patient serum",

author = "Lan Wang and Wenjing Qin and Ding Chen and Nanxiang Wang and Congcong Zhang and Zhijie Fang and Bin Fang and Wei Du and Naidi Yang and Qiong Wu and Bo Peng and Lin Li",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = jan,

doi = "10.1016/j.rechem.2021.100103",

language = "英语",

volume = "3",

journal = "Results in Chemistry",

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TY - JOUR

T1 - Design, synthesis and application of fluorogenic probe for detecting L-asparaginase in serum samples

AU - Wang, Lan

AU - Qin, Wenjing

AU - Chen, Ding

AU - Wang, Nanxiang

AU - Zhang, Congcong

AU - Fang, Zhijie

AU - Fang, Bin

AU - Du, Wei

AU - Yang, Naidi

AU - Wu, Qiong

AU - Peng, Bo

AU - Li, Lin

PY - 2021/1

Y1 - 2021/1

N2 - Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro L-asparaginase detection. TPAN-Asn exhibited selective and robust response to L-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of L-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of L-asparaginase-based cancer therapy.

AB - Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro L-asparaginase detection. TPAN-Asn exhibited selective and robust response to L-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of L-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of L-asparaginase-based cancer therapy.

KW - Fluorogenic probe

KW - In vitro assay

KW - L-asparaginase

KW - Patient serum

UR - https://www.scopus.com/pages/publications/85101072197

U2 - 10.1016/j.rechem.2021.100103

DO - 10.1016/j.rechem.2021.100103

M3 - 文章

AN - SCOPUS:85101072197

SN - 2211-7156

VL - 3

JO - Results in Chemistry

JF - Results in Chemistry

M1 - 100103

ER -

Design, synthesis and application of fluorogenic probe for detecting L-asparaginase in serum samples

Abstract

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Keywords

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