Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

Xue Li; Lei Tian; Zafar Ali; Wenyan Wang; Qiuyu Zhang

doi:10.1007/s10853-017-1581-9

Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

Xue Li
, Lei Tian
, Zafar Ali
, Wenyan Wang
, Qiuyu Zhang

School of Chemistry and Chemical Engineering

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Micron-sized flower-like porous Fe₃O₄ microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe₃O₄@SiO₂–NH₂/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.

Original language	English
Pages (from-to)	937-947
Number of pages	11
Journal	Journal of Materials Science
Volume	53
Issue number	2
DOIs	https://doi.org/10.1007/s10853-017-1581-9
State	Published - 1 Jan 2018

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title = "Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization",

abstract = "Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14\% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.",

author = "Xue Li and Lei Tian and Zafar Ali and Wenyan Wang and Qiuyu Zhang",

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T1 - Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

AU - Li, Xue

AU - Tian, Lei

AU - Ali, Zafar

AU - Wang, Wenyan

AU - Zhang, Qiuyu

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.

AB - Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.

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Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization

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