Comparison of the quality of protein crystals grown by CLPC seeds method

Jin Li; Er Kai Yan; Yue Liu; Zi Qing Wu; Ya Li Liu; Hai Hou; Chen Yan Zhang; Qin Qin Lu; Xu Dong Deng; Da Chuan Yin

doi:10.3390/cryst9100501

Comparison of the quality of protein crystals grown by CLPC seeds method

Jin Li
, Er Kai Yan
, Yue Liu
, Zi Qing Wu
, Ya Li Liu
, Hai Hou
, Chen Yan Zhang
, Qin Qin Lu
, Xu Dong Deng
, Da Chuan Yin

School of life sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

Original language	English
Article number	501
Journal	Crystals
Volume	9
Issue number	10
DOIs	https://doi.org/10.3390/cryst9100501
State	Published - Oct 2019

Keywords

CLPC seeds
Crystal quality
Mosaicity
Resolution

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10.3390/cryst9100501

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@article{d5adde50ba594885ad19f3a0cf183e27,

title = "Comparison of the quality of protein crystals grown by CLPC seeds method",

abstract = "We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.",

keywords = "CLPC seeds, Crystal quality, Mosaicity, Resolution",

author = "Jin Li and Yan, \{Er Kai\} and Yue Liu and Wu, \{Zi Qing\} and Liu, \{Ya Li\} and Hai Hou and Zhang, \{Chen Yan\} and Lu, \{Qin Qin\} and Deng, \{Xu Dong\} and Yin, \{Da Chuan\}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = oct,

doi = "10.3390/cryst9100501",

language = "英语",

volume = "9",

journal = "Crystals",

issn = "2073-4352",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "10",

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TY - JOUR

T1 - Comparison of the quality of protein crystals grown by CLPC seeds method

AU - Li, Jin

AU - Yan, Er Kai

AU - Liu, Yue

AU - Wu, Zi Qing

AU - Liu, Ya Li

AU - Hou, Hai

AU - Zhang, Chen Yan

AU - Lu, Qin Qin

AU - Deng, Xu Dong

AU - Yin, Da Chuan

PY - 2019/10

Y1 - 2019/10

N2 - We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

AB - We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

KW - CLPC seeds

KW - Crystal quality

KW - Mosaicity

KW - Resolution

UR - https://www.scopus.com/pages/publications/85073423558

U2 - 10.3390/cryst9100501

DO - 10.3390/cryst9100501

M3 - 文章

AN - SCOPUS:85073423558

SN - 2073-4352

VL - 9

JO - Crystals

JF - Crystals

IS - 10

M1 - 501

ER -

Comparison of the quality of protein crystals grown by CLPC seeds method

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Keywords

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