TY - JOUR
T1 - Bifacial effects of engineering tumour cell-derived exosomes on human natural killer cells
AU - Li, Qi
AU - Huang, Qiuping
AU - Huyan, Ting
AU - Wang, Yilin
AU - Huang, Qingsheng
AU - Shi, Junling
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/2/15
Y1 - 2018/2/15
N2 - Extracellular vesicles (EVs) are nano vesicular structures that are secreted by almost all kinds of cells. Exosomes are small EVs derived from endosomes, with a diameter between 30–100 nm. Tumour-derived exosomes carry many molecules and factors from tumour cells. These exosomes are recognized and taken up by immunocytes. However, tumour-derived exosomes can not only suppress immune cell functions but also help tumours escape immune surveillance in the tumour microenvironment. The present work investigated the effect of exosomes derived from genetical modified K562 cells (GMK cells), which express IL-15, IL-18 and 4-1BBL (TNFSF9) on their surface. The results showed that these GME exosomes, carrying IL-15, IL-18 and 4-1BBL proteins similar to their host cells, could activate NK cells, increase the cytotoxicity of NK cells on some tumour cells in a short treatment (4 h) and promote NK cells proliferation. However, with an extended treatment time (48 h), these exosomes could inhibite the cytotoxicity of NK cells by inhibiting activated receptor expression on NK cells. These results indicated the bifacial effects of GMK exosomes on NK cells, which will be helpful to explore the possibility of using transformed exosomes as an anti-tumour immune vaccine or a therapeutic tool in future.
AB - Extracellular vesicles (EVs) are nano vesicular structures that are secreted by almost all kinds of cells. Exosomes are small EVs derived from endosomes, with a diameter between 30–100 nm. Tumour-derived exosomes carry many molecules and factors from tumour cells. These exosomes are recognized and taken up by immunocytes. However, tumour-derived exosomes can not only suppress immune cell functions but also help tumours escape immune surveillance in the tumour microenvironment. The present work investigated the effect of exosomes derived from genetical modified K562 cells (GMK cells), which express IL-15, IL-18 and 4-1BBL (TNFSF9) on their surface. The results showed that these GME exosomes, carrying IL-15, IL-18 and 4-1BBL proteins similar to their host cells, could activate NK cells, increase the cytotoxicity of NK cells on some tumour cells in a short treatment (4 h) and promote NK cells proliferation. However, with an extended treatment time (48 h), these exosomes could inhibite the cytotoxicity of NK cells by inhibiting activated receptor expression on NK cells. These results indicated the bifacial effects of GMK exosomes on NK cells, which will be helpful to explore the possibility of using transformed exosomes as an anti-tumour immune vaccine or a therapeutic tool in future.
KW - Bifacial effects
KW - Exosomes
KW - Extracellular vesicles
KW - Genetically modified K562 cells
KW - Natural killer cells
UR - http://www.scopus.com/inward/record.url?scp=85039908802&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2017.12.005
DO - 10.1016/j.yexcr.2017.12.005
M3 - 文章
C2 - 29269076
AN - SCOPUS:85039908802
SN - 0014-4827
VL - 363
SP - 141
EP - 150
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -
