Adsorption and desorption properties of macroporous resin to pinoresinol diglucoside in liquid culture

Four macroporous resins, including of S-8, AB-8, NKA-II and NKA-9, were studied on separation and purification of Pinoresinol Diglucoside (PDG) in the liquid culture of Phomopsis sp., a strain isolated from Eucommia ulmoides Oliv. The experimental results showed that S-8 resin could achieve better adsorption and desorption performance than others. With S-8 resin, equilibrium and kinetics of static adsorption of PDG were studies in detail and the effects of temperature and pH on the adsorption performance were also discussed. The dynamic adsorption and desorption conditions of S-8 resin for PDG were optimized. The results indicated that isothermal sorption process of PDG on S-8 resin could be well described by Langmuir equation, and the adsorption rate of the initial stage and the later stage were controlled by liquid film diffusion and inner-particle diffusion, respectively. The optimum dynamic adsorption conditions for PDG were as follows: PDG concentration, 0.195mg/mL; pH 9; adsorption temperature, 20°C; flow rate, lBV/h; and saturated adsorption amount of PDG, 20BV. The optimum desorption conditions were as follows: eluant, 30% ethanol aqueous solution (v/v); the amount of elution, 6BV and eluant flowing rate, 1BV/h. In these techniques, high recovery efficiency of PDG as much as 89.8% was obtained.