A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

Xingwei Chen; Jiajia Xu; Fengtai Suo; Changmin Yu; Duoteng Zhang; Jian Chen; Qiong Wu; Su Jing; Lin Li; Wei Huang

doi:10.1016/j.saa.2019.117949

A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

Xingwei Chen
, Jiajia Xu
, Fengtai Suo
, Changmin Yu
, Duoteng Zhang
, Jian Chen
, Qiong Wu
, Su Jing
, Lin Li
, Wei Huang

Institute of Flexible Electronics

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn²⁺. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn²⁺ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn²⁺ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn²⁺ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn²⁺ in the living cells and zebrafishes.

Original language	English
Article number	117949
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	229
DOIs	https://doi.org/10.1016/j.saa.2019.117949
State	Published - 15 Mar 2020

Keywords

High-throughput
Imaging
Paper-based devices
Point-of-care testing
Zinc ions

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10.1016/j.saa.2019.117949

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Chen, X., Xu, J., Suo, F., Yu, C., Zhang, D., Chen, J., Wu, Q., Jing, S., Li, L., & Huang, W. (2020). A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 229, Article 117949. https://doi.org/10.1016/j.saa.2019.117949

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title = "A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes",

abstract = "The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.",

keywords = "High-throughput, Imaging, Paper-based devices, Point-of-care testing, Zinc ions",

author = "Xingwei Chen and Jiajia Xu and Fengtai Suo and Changmin Yu and Duoteng Zhang and Jian Chen and Qiong Wu and Su Jing and Lin Li and Wei Huang",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2020",

month = mar,

day = "15",

doi = "10.1016/j.saa.2019.117949",

language = "英语",

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Chen, X, Xu, J, Suo, F, Yu, C, Zhang, D, Chen, J, Wu, Q, Jing, S, Li, L & Huang, W 2020, 'A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes', Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 229, 117949. https://doi.org/10.1016/j.saa.2019.117949

A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes. / Chen, Xingwei; Xu, Jiajia; Suo, Fengtai et al.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 229, 117949, 15.03.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

AU - Chen, Xingwei

AU - Xu, Jiajia

AU - Suo, Fengtai

AU - Yu, Changmin

AU - Zhang, Duoteng

AU - Chen, Jian

AU - Wu, Qiong

AU - Jing, Su

AU - Li, Lin

AU - Huang, Wei

PY - 2020/3/15

Y1 - 2020/3/15

N2 - The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.

AB - The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.

KW - High-throughput

KW - Imaging

KW - Paper-based devices

KW - Point-of-care testing

KW - Zinc ions

UR - https://www.scopus.com/pages/publications/85076474666

U2 - 10.1016/j.saa.2019.117949

DO - 10.1016/j.saa.2019.117949

M3 - 文章

C2 - 31864152

AN - SCOPUS:85076474666

SN - 1386-1425

VL - 229

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

M1 - 117949

ER -

A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

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Keywords

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