A feasible electrochemical biosensor for determination of glucose based on Prussian blue – Enzyme aggregates cascade catalytic system

The coral-like gold micro/nanostructures were formed onto carbon cloth followed by a Prussian blue (PB) electrochemical deposition to construct a highly sensitive H₂O₂ biosensor. The SEM image of PB/Au/CC showed the coral-like gold morphology, and EDS and XPS tests also further confirmed the successful loading of Au and PB. The electrochemical tests of PB/Au/CC displayed the electrode possessed excellent performance in sensing H₂O₂, which was quantified in the linear range from 0.002 to 13.97 mM at an applied potential of −0.05 V, with a sensitivity of 454.97 μA mM⁻¹ cm⁻² and a detection limit of 0.5 μM (S/N = 3). And then a convenient sensing platform was established via the cross-linking enzyme aggregates method, using PB as the mediator to realize the construction of glucose BIOSENSOR GOx_EA@PB/Au/CC. The biosensor responded to glucose in the sensitivity of 70.76 μA mM⁻¹ cm⁻² within the linear range from 0.05 to 3.15 mM with a detection limit of 10 μM. The sensitivity was much higher than the electrode constructed by the cross-linking enzyme method (GOx@PB/Au/CC), and it was also highly selective, reproducible, and stable. Besides, the proposed biosensor was successfully applied to the glucose determination in real human serum samples, which proved its practicability.