A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Hai Hou; Bo Wang; Shan Yang Hu; Jing Zhang Wang; Peng Fei Zhu; Yue Liu; Meng Ying Wang; Da Chuan Yin

doi:10.1039/c5ce00551e

A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Hai Hou
, Bo Wang
, Shan Yang Hu
, Jing Zhang Wang
, Peng Fei Zhu
, Yue Liu
, Meng Ying Wang
, Da Chuan Yin

School of life sciences

Northwestern Polytechnical University Xian
Hebei University of Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

Original language	English
Pages (from-to)	5365-5371
Number of pages	7
Journal	CrystEngComm
Volume	17
Issue number	29
DOIs	https://doi.org/10.1039/c5ce00551e
State	Published - 7 Aug 2015

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title = "A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods",

abstract = "We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.",

author = "Hai Hou and Bo Wang and Hu, \{Shan Yang\} and Wang, \{Jing Zhang\} and Zhu, \{Peng Fei\} and Yue Liu and Wang, \{Meng Ying\} and Yin, \{Da Chuan\}",

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AU - Hou, Hai

AU - Wang, Bo

AU - Hu, Shan Yang

AU - Wang, Jing Zhang

AU - Zhu, Peng Fei

AU - Liu, Yue

AU - Wang, Meng Ying

AU - Yin, Da Chuan

PY - 2015/8/7

Y1 - 2015/8/7

N2 - We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

AB - We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

UR - https://www.scopus.com/pages/publications/84937053863

U2 - 10.1039/c5ce00551e

DO - 10.1039/c5ce00551e

M3 - 文章

AN - SCOPUS:84937053863

SN - 1466-8033

VL - 17

SP - 5365

EP - 5371

JO - CrystEngComm

JF - CrystEngComm

IS - 29

ER -

A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Abstract

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