A novel electrochemical biosensor for detection of cholesterol

We report on a highly sensitive electrochemical biosensor for determination of cholesterol. The biosensor was fabricated by co-immobilizing bi-enzymes, cholesterol oxidase (ChOx), and horseradish peroxidase (HRP). Voltammetric technique such as cyclic voltammetry and impedance experiment were used to study the characterization of modified electrode step by step. The developed sensor is cheap, disposable, portable and exhibits higher sensitivity. The biosensor expressed a wide linear range up to 300 mg dL^–1 in a physiological condition (pH 7.0), with a correlation coefficient of 0.9969. A sensitivity of 13.28 μA mg^–1 dL cm⁻² which makes it very promising for the clinical determination of cholesterol.